Extracellular matrix deposition assays
Interpret extracellular matrix results by separating newly deposited material, pre-existing scaffold, cell numbers and matrix organisation.
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Collagen measurement methods in cell studies
Distinguish collagen-associated RNA, soluble protein, deposited material and breakdown measurements before comparing cell-study results.
Read the article →Enzyme inhibition assays using peptide substrates
Read an enzyme-inhibition result by identifying the peptide substrate, reaction measurement, assay conditions and checks for misleading signals.
Read the article →Protease susceptibility assays
Interpret peptide cleavage experiments by checking the protease system, intact-parent measurement, fragments and time-dependent comparison.
Read the article →Antimicrobial peptide assay endpoints
Read antimicrobial peptide results by distinguishing growth inhibition, viable-count changes, concentration limits and clinical interpretation.
Read the article →Haemolysis assays in peptide research
Understand red-cell haemoglobin release, control-relative percentages and the limits of haemolysis-based safety claims in peptide studies.
Read the article →Immune-cell chemotaxis assays
Distinguish directed immune-cell migration from faster movement, endpoint accumulation and changes in the chemical gradient.
Read the article →Neurite outgrowth assays
Interpret neuronal morphology by separating neurite length, branching, cell survival and evidence of functional neural connections.
Read the article →Vehicle effects in peptide assay systems
Identify changes caused by the surrounding solvent or formulation and distinguish a matched vehicle comparison from an untreated baseline.
Read the article →Peptide adsorption as an assay confounder
Recognise how peptide loss to experimental surfaces can separate nominal concentration from measured exposure and complicate assay interpretation.
Read the article →Serum binding and apparent peptide activity
Distinguish total peptide from unbound peptide when comparing activity in media with different serum or protein content.
Read the article →Proteolysis during an assay
Assess whether a peptide experiment measures the starting molecule, cleavage products or an unresolved mixture over time.
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