NAD+ identity: beta-NAD+, NADH, formula and certificate checks

Read NAD+ names, CAS number and charged molecular forms, and distinguish beta-NAD+ assay results from NADH and total-content measurements.

Substantially revised on 3 October 2026 to focus on molecular identity, naming, chemistry, testing and documentation.

NAD+ is oxidised nicotinamide adenine dinucleotide, a nucleotide coenzyme. The supplied certificate names beta-NAD+ and reports NADH separately. Reading those entries starts with the chemical form each name represents.

NAD+ names and the represented molecular form

ChEBI CHEBI:15846 names the oxidised form and records CAS 53-84-9. Its displayed structure has net charge +1. The formula and average mass below belong to that particular representation; a different protonation state or added counterion needs its own specification.ChEBI: NAD+, CHEBI:15846 (opens in a new tab)

ChEBI CHEBI:15846 identity reference
FieldReference or document detail
NamesNAD+; nicotinamide adenine dinucleotide; nadide; DPN
CAS number53-84-9
Molecular formula in this recordC21H28N7O14P2
Net charge in this record+1
Average molecular mass664.438 Da
Peptide sequenceNot applicable: nucleotide coenzyme
ChEBI: NAD+, CHEBI:15846 (opens in a new tab)

The supplied report specifies oxidised beta-nicotinamide adenine dinucleotide and comparison against a beta-NAD+ reference. It does not specify a complete salt or hydration formula, so the database representation should remain labelled as a reference rather than a full batch formula.NAD+ 1000 mg report: MA-NAD-0005 (opens in a new tab)ChEBI: NAD+, CHEBI:15846 (opens in a new tab)

NAD+ and NADH are different redox forms

ChEBI records NADH as the reduced form. Its separate NAD(1−) and NADH(2−) records show why net charge and redox identity must be read independently: a minus sign in the charge field does not by itself identify NADH.ChEBI: NADH, CHEBI:16908 (opens in a new tab)ChEBI: NAD(1−), CHEBI:57540 (opens in a new tab)ChEBI: NADH(2−), CHEBI:57945 (opens in a new tab)

An explicitly hypothetical mixture of 90 units NAD+ and 10 units NADH totals 100 units and has an NAD+/NADH ratio of 9. A 50:50 mixture has the same total but a ratio of 1. This arithmetic illustrates why both measured forms are needed for the ratio.ChEBI: NAD+, CHEBI:15846 (opens in a new tab)ChEBI: NADH, CHEBI:16908 (opens in a new tab)

Read the coenzyme-specific certificate

Report MA-NAD-0005, revision 01, identifies lot NAD-05-9NW. It reports beta-NAD+ chromatographic assay of 99.38%, NADH of 0.14%, ADP-ribose of 0.18% and measured NAD+ content of 1002.00 mg. These are distinct report fields, not interchangeable ways of expressing the same quantity.NAD+ 1000 mg report: MA-NAD-0005 (opens in a new tab)

Preserve the analyte names, analytical methods and units when transcribing the report. A percentage based on chromatographic area should remain labelled that way; the milligram content belongs in its own entry.NAD+ 1000 mg report: MA-NAD-0005 (opens in a new tab)

Product and certificate checks

Sources and further detail

  1. NAD+ 1000 mg report: MA-NAD-0005 (opens in a new tab)

    Supplied PDF re-read on 3 October 2026. Identity wording and document-specific results are transcribed; this is not independent authentication.

  2. ChEBI: NAD+, CHEBI:15846 (opens in a new tab)

    Identity, CAS mapping, +1 charge, formula and average mass rechecked on 3 October 2026; the represented ionic form is explicit.

  3. ChEBI: NADH, CHEBI:16908 (opens in a new tab)

    Existing donor source for reduced-form identity.

  4. ChEBI: NAD(1−), CHEBI:57540 (opens in a new tab)

    Existing donor source for phosphate deprotonation and the represented net charge.

  5. ChEBI: NADH(2−), CHEBI:57945 (opens in a new tab)

    Existing donor source for the reduced dianion.

Revised 3 October 2026 using the existing source dossier and the specified primary identity records. Report values describe the linked samples. Products are for laboratory research use only.

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